Nucleotide sequence of a full-length cDNA for arylamine N-acetyltransferase from rabbit liver.

A full-length cDNA clone encoding rabbit liver arylamine *• N-acetyltransferase (NAT; EC 2.3.1.5) was isolated from a Xgtll [ expression library by concomitant screening with an oligonucleof tide deduced from partial amino acid sequences (1) and with antibodies raised against the purified protein. The nucleotide p sequence of the clone, designated rnat, consists of 1102 nucleotides and contains a single open reading frame of 870 nucleotides A encoding a 290 amino acid protein (Mr 33,655 Da) with 14 of 16 tryptic peptide sequences recently determined from purified * rabbit liver NAT (1). The rnat cDNA shows 61% and 46% sequence homology at the nucleotide and deduced amino acid level, |f respectively, to those of NAT from chicken liver (2).